Affinity of linker histones for chromatin in situ analyzed using DAPI as a cytochemical probe
نویسندگان
چکیده
منابع مشابه
Linker histones incorporation maintains chromatin fiber plasticity.
Genomic DNA in eukaryotic cells is organized in supercoiled chromatin fibers, which undergo dynamic changes during such DNA metabolic processes as transcription or replication. Indeed, DNA-translocating enzymes like polymerases produce physical constraints in vivo. We used single-molecule micromanipulation by magnetic tweezers to study the response of chromatin to mechanical constraints in the ...
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چکیده ندارد.
15 صفحه اولDAPI: a DNA-specific fluorescent probe.
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are des...
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Patrick Cramer is a professor of Biochemistry at the Ludwig Maximilians University of Munich, Germany, and head of the Gene Center Munich. He is a structural biologist who has contributed to our understanding of the structure and function of RNAP II from yeast and its associated factors. In a recent study, his laboratory described the structure of RNAP II in complex with the initiation factor T...
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DNA complementary to prolactin-specific sequences was prepared using a method which did not require purification of the prolactin mRNA. cDNA was synthesized using total cytoplasmic mRNA from a prolactinproducing rat pituitary cell line (GH,) as a template for avian myeloblastosis virus reverse transcriptase. A cDNA species 840 nucleotides in length was purified by electrophoresis on formamide p...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2000
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(20000501)40:1<1::aid-cyto1>3.3.co;2-7